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Liquid Papain 80,000 U
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Liquid Papain 80,000 U

Liquid Papain 80,000 U

Papain is a biological enzyme preparation extracted from unripe papaya fruit through biotechnology. Composed of 212 amino acids, it has a molecular weight of 21,000. This enzyme belongs to the class of endopeptidases containing a thiol group (-SH), exhibiting both protease and esterase activities. It exhibits broad specificity, demonstrating potent enzymatic hydrolysis capabilities toward animal and plant proteins, peptides, esters, amides, and other substances. Additionally, papain possesses synthetic capabilities, enabling it to reassemble protein hydrolysis products into protein-like substances. This property can be utilized to enhance the nutritional value and physical properties of animal and plant proteins.
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Product Details ofLiquid Papain 80,000 U

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Question

What is papain good for?

 

Beer and beverage clarification: prevents haze caused by proteins.

Meat tenderizing: softens meat by breaking down protein.

Food processing: produces protein hydrolysates, improves dough and cheese texture.

Cosmetics: used in exfoliating and skin-smoothing products.

Industrial use: applied in leather dehairing and textile finishing.

 

Product-information

Product name:

Papain

source:

Papaya Latex

Technology :

Natural extraction

Appearance:

Light yellow liquid

Product odor:

Free of offensive odors

Enzyme activity:

80,000 u/g or customized

MOQ:

1L

Packing:

5L/drum; 25L/drum

Storage:

In a cool and dry environment away from light.

Shelf life:

12 Months under room temperature

18 Months under 15℃

24 Months under 4℃

Optimum environment:

40℃~60℃; pH 6.0 ~ 7.0

Other influencing factors:

Oxidation inhibitor; Reducing substances, like active ion Ca2+, Mg2+, Na+; K+ ect.

Certificate:

ISO9001,FSSC22000

Recommended dosage:

Suggested addition amount is 0.5% (5 ml of Papain per liter of beer), depending on the type, concentration, reaction conditions, and other factors of the substrate and production situation.

 

Specification

Product Name: Papain

ITEMS OF ANALYSIS

SPECIFICATIONS

Appearance

Light yellow liquid

Odor

Free of offensive odors

Activity of enzyme

NLT 80,000 u/g

Loss on drying

NMT 5.0%

Heavy metals

<20.0mg/kg

Pb

<2.0mg/kg

As

<2.0mg/kg

Total Plate Count

<10,000 cfu/g

E.Coli

Negative

Salmonella

None detected in 10g

Yeast and Mould

<100cfu/g

Storage: In a cool and dry environment away from light.

Shelf Life: It can be stored for 24 months in an airtight container at 4℃, 18 months at 15℃, and 12 months at room temperature.

application

1.Beer clarification:

Papain acts as a natural protease in beer clarification, breaking down soluble proteins and polypeptides that cause turbidity. It improves beer's clarity and stability, ensuring a bright appearance without affecting flavor, with dosage adjusted based on raw material properties and brewing conditions.

 

2.Vinegar clarification:

In vinegar clarification, Papain hydrolyzes haze-forming proteins and colloidal substances from raw materials like grains or fruits. It enhances vinegar's transparency and shelf life, maintaining its original taste and nutritional value while simplifying the filtration process in industrial production.

 

3.Sediment removal:

For sediment removal in liquid products (e.g., beverages, condiments), Papain decomposes insoluble protein aggregates and suspended particles. It prevents sediment formation during storage, optimizes product quality and consumer experience, and works efficiently under mild processing conditions.

 

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1.Thermal Stability: Papain is stable at temperatures below 70 °C. Its enzymatic activity reaches optimal levels within the range of 40–60 °C, allowing efficient protein hydrolysis under typical processing conditions.

 

2.pH Stability: Papain remains effective over a broad pH range of 3.0–9.5. The enzyme exhibits optimal activity in a near-neutral environment, with a preferred pH of 6.0–7.0, making it suitable for various food and beverage applications.

 

3.Natural Source: Papain is a natural enzyme preparation obtained from the unripe fruit pulp of papaya through biotechnological extraction.

 

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Q1:What fruit has the most papain?
A1:1.Unripe papaya: Contains the highest concentration of active papain in both the pulp and the latex (the milky sap).

2.Papaya latex: The latex collected from cuts in the green fruit is especially rich in papain and is commonly used for industrial extraction.

3.Ripe papaya: Contains much less active papain because the enzyme decreases as the fruit ripens.

 

Q2: How does papain clarify beer?
A2: Papain clarifies beer by hydrolyzing haze-forming proteins, which would otherwise bind with polyphenols and cause turbidity, especially at low temperatures. By breaking these proteins into smaller peptides and amino acids, papain prevents cloudiness, accelerates filtration, and helps maintain foam stability without affecting the beer's taste. It is typically added during brewing or after fermentation in controlled amounts to achieve optimal clarity.

 

Q3: Which is better, papain or bromelain?
A3: Papain and bromelain are both proteolytic enzymes, but their suitability depends on the application. Papain is preferred for beverage and beer clarification due to mild taste and effective protein hydrolysis, while bromelain is often used for meat tenderizing and nutraceuticals because it works well on animal proteins and tolerates higher temperatures.

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