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Alpha Acetolactate Decarboxylase
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Alpha Acetolactate Decarboxylase

Alpha Acetolactate Decarboxylase

Alpha-Acetolactate Decarboxylase (α-ALDC) is a highly specific enzyme preparation used primarily in the brewing industry. It is produced through the controlled fermentation of a non-pathogenic, non-genetically modified microbial strain. This enzyme directly targets and converts alpha-acetolactate, a key precursor to diacetyl, thereby significantly reducing or eliminating diacetyl formation during fermentation. This results in a faster maturation time and a more consistent, high-quality final product.
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Product Details ofAlpha Acetolactate Decarboxylase

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Question

What does Aldc do for beer?

 

Alpha Acetolactate Decarboxylase(ALDC) can solve the problem of "bready odor" in beer fermentation: During the fermentation process, yeast produces α-acetylated lactic acid, which naturally converts into diacetyl (a harmful substance with a spoiled rice smell) and requires a long time to be restored and removed. This results in a fermentation cycle of up to 7-10 days. By adding ALDC, α-acetylated lactic acid can be directly converted into odorless acetic acid, shortening the fermentation cycle to 3-5 days, while reducing diacetyl residue (conforming to ≤0.1mg/L), improving the stability of beer taste, and also reducing energy consumption and equipment occupancy rate.

 

Product-information

Product name:

Alpha Acetolactate Decarboxylase

Appearance:

Light yellow liquid

Product odor:

Slight odor of fermentation

Ingredients:

Alpha Acetolactate Decarboxylase, Maltodextrin

Enzyme activity:

1500u/ml or customized

MOQ:

1kg

Package:

1kg/bottle, 25kg/drum

Shelf Life:

12 Months under room temperature

18 Months under 15 oC

24 Months under 0 ~ 4 oC

Optimum environment:

4℃~70℃; pH 3.5 ~ 7.5

Storage:

In cool dry place , away from light

Recommended dosage:

Suggested addition amount is 5-500g per ton, depending on the type, concentration, reaction conditions, and other factors of the substrate and production situation

 

Mechanism of action

 

Alpha-Acetolactate Decarboxylase (α-ALDC) specifically recognizes the substrate α-acetyl lactate. Through a "decarboxylation reaction", it removes one carboxyl group from the molecule and directly converts it into acetoacetic acid, thus skipping the step of generating diacetyl - not only avoiding the unpleasant odors that diacetyl brings to beer, food, etc., but also accelerating the maturation of product flavor without waiting for the subsequent natural decomposition of diacetyl.

 

Specification

Product name

Alpha-Acetolactate Decarboxylase (α-ALDC)

Form:

Liquid

CAS #.:

9001-22-3

Enzyme Activity

NLT 2000u/g or customized

Item

Specifciatiom

Appearance:

Light brown to brown liquid; There is no obvious sedimentation or stratification, and there is a slight residual aroma of the culture medium.

Activity of enzyme:

NLT 2,000 u/g

Arsenic:

NMT 3.00 mg/kg

Lead:

NMT 5.00 mg/kg

Colonies number:

NMT 50,000 cfu/g

Coliform bacteria:

NMT 30 cfu/g

Escherichia Coli:

Negative

Salmonella:

Negative/25g

 

application

The core application of α-acetyl lactate decarboxylase (ALDC) is mainly concentrated in the food fermentation and related industrial fields, with the beer industry being the core. The specific application scenarios and values are as follows:

The beer/malt beverage industry (the main application) solves the problem of "diacetyl odor" in beer fermentation: During fermentation, yeast produces α-acetyl lactate, which naturally converts into diacetyl (a harmful substance with a spoiled rice smell) and then requires a long time to be restored and removed, resulting in a fermentation cycle of 7-10 days. Adding ALDC can directly convert α-acetyl lactate into odorless acetic acid, shortening the fermentation cycle to 3-5 days, while reducing diacetyl residue (conforming to ≤0.1mg/L), improving the stability of beer taste, and also reducing energy consumption and equipment occupancy rate.

Other fermented foods/drinks are applicable to wine, fruit wine, lactic acid bacteria beverages, fermented milk, etc.: It avoids the accumulation of diacetyl during fermentation, accelerates product maturation, and improves flavor consistency (such as reducing the "rancid taste" in lactic acid bacteria beverages and maintaining pure fruit aroma in fruit wine).

In the field of biochemistry, it is used for amino acid (such as valine, leucine) fermentation production: α-acetyl lactate is a by-product of these amino acid synthesis, and ALDC can degrade the by-product, reduce the consumption of metabolic pathways, and increase the yield and purity of the target amino acid.

 

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Drastically Reduced production Time: The most significant benefit. By preventing diacetyl formation, the need for a lengthy lagering or diacetyl rest is eliminated. This can reduce total production time from weeks to days, increasing brewery throughput.

Improved Fermentation Control: Ensures diacetyl levels remain below the taste threshold, resulting in a more predictable and controllable fermentation process.

Enhanced Product Consistency & Quality: Eliminates the risk of diacetyl-related off-flavors in the final product, guaranteeing a clean, crisp profile batch after batch.

Increased Tank Efficiency: Faster turnaround times mean fermentation tanks are free for more batches per year, optimizing capital investment.

Energy Savings: Shorter maturation times lead to significant savings in energy costs for tank refrigeration.

Flexibility: Particularly beneficial for breweries producing lager-style beers under pressure to meet high demand, and for ale brewers looking to guarantee a diacetyl-free product.

 

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Q1: Does using α-ALDC affect the final flavor of my beer?
A: No, when used correctly, it improves flavor stability by preventing the diacetyl off-flavor. The end product, acetoin, has a much higher flavor threshold and is considered neutral, contributing no undesirable flavors.

 

Q2: Is this enzyme considered "natural" for labeling purposes?
A: Regulations vary by country. In many regions, enzymes are considered processing aids and do not need to be declared on the final product label because they are inactivated and removed. Always check local regulatory guidelines.

 

Q3: Can I use α-ALDC to fix a beer that already has a diacetyl problem?
A: No. α-ALDC works by preventing diacetyl formation by acting on its precursor. It cannot convert existing diacetyl back into acetoin. For a beer with high diacetyl, a traditional diacetyl rest (if yeast is still active) is the only solution.

 

 

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